The Legionella micdadei flagellin: expression in Escherichia coli K 12 and DNA sequence of the gene

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The Legionella micdadei flagellin : expression in Escherichia coli K 12 and DNA sequence of the gene. / Bangsborg, Jette Marie; Hindersson, P; Shand, G; Høiby, N.

In: A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica, Vol. 103, No. 12, 1995, p. 869-77.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bangsborg, JM, Hindersson, P, Shand, G & Høiby, N 1995, 'The Legionella micdadei flagellin: expression in Escherichia coli K 12 and DNA sequence of the gene', A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica, vol. 103, no. 12, pp. 869-77.

APA

Bangsborg, J. M., Hindersson, P., Shand, G., & Høiby, N. (1995). The Legionella micdadei flagellin: expression in Escherichia coli K 12 and DNA sequence of the gene. A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica, 103(12), 869-77.

Vancouver

Bangsborg JM, Hindersson P, Shand G, Høiby N. The Legionella micdadei flagellin: expression in Escherichia coli K 12 and DNA sequence of the gene. A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica. 1995;103(12):869-77.

Author

Bangsborg, Jette Marie ; Hindersson, P ; Shand, G ; Høiby, N. / The Legionella micdadei flagellin : expression in Escherichia coli K 12 and DNA sequence of the gene. In: A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica. 1995 ; Vol. 103, No. 12. pp. 869-77.

Bibtex

@article{b515ad250a0842d39890722adafecdc1,
title = "The Legionella micdadei flagellin: expression in Escherichia coli K 12 and DNA sequence of the gene",
abstract = "To study the structure and function of the Legionella flagellum, we screened a genomic L. micdadei library in Escherichia coli for expression of the flagellin (Fla) subunit. One recombinant clone, JM105 (pHI5588), producing a truncated Fla protein of 40.5 kDa was identified. The plasmid pHI5588 carried a L. micdadei DNA insert of 5 kb, containing ca 95% of the fla gene. The complete DNA sequence of the L. micdadei fla gene was obtained by combining sequence data from pHI5588 with results using a polymerase chain reaction-based system for genome walking (vectorette PCR). The L. micdadei fla gene shared a high degree of homology with other flagellin genes in the amino- and carboxy termini, whereas the central region was found to be nonconserved. The fla sequence will facilitate the cloning of Fla proteins from other Legionella species and the study of flagella in the pathogenesis of Legionnaires' disease.",
keywords = "Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Bacterial, Escherichia coli, Flagellin, Legionella, Molecular Sequence Data",
author = "Bangsborg, {Jette Marie} and P Hindersson and G Shand and N H{\o}iby",
year = "1995",
language = "English",
volume = "103",
pages = "869--77",
journal = "A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica",
issn = "0903-4641",
publisher = "Wiley Online",
number = "12",

}

RIS

TY - JOUR

T1 - The Legionella micdadei flagellin

T2 - expression in Escherichia coli K 12 and DNA sequence of the gene

AU - Bangsborg, Jette Marie

AU - Hindersson, P

AU - Shand, G

AU - Høiby, N

PY - 1995

Y1 - 1995

N2 - To study the structure and function of the Legionella flagellum, we screened a genomic L. micdadei library in Escherichia coli for expression of the flagellin (Fla) subunit. One recombinant clone, JM105 (pHI5588), producing a truncated Fla protein of 40.5 kDa was identified. The plasmid pHI5588 carried a L. micdadei DNA insert of 5 kb, containing ca 95% of the fla gene. The complete DNA sequence of the L. micdadei fla gene was obtained by combining sequence data from pHI5588 with results using a polymerase chain reaction-based system for genome walking (vectorette PCR). The L. micdadei fla gene shared a high degree of homology with other flagellin genes in the amino- and carboxy termini, whereas the central region was found to be nonconserved. The fla sequence will facilitate the cloning of Fla proteins from other Legionella species and the study of flagella in the pathogenesis of Legionnaires' disease.

AB - To study the structure and function of the Legionella flagellum, we screened a genomic L. micdadei library in Escherichia coli for expression of the flagellin (Fla) subunit. One recombinant clone, JM105 (pHI5588), producing a truncated Fla protein of 40.5 kDa was identified. The plasmid pHI5588 carried a L. micdadei DNA insert of 5 kb, containing ca 95% of the fla gene. The complete DNA sequence of the L. micdadei fla gene was obtained by combining sequence data from pHI5588 with results using a polymerase chain reaction-based system for genome walking (vectorette PCR). The L. micdadei fla gene shared a high degree of homology with other flagellin genes in the amino- and carboxy termini, whereas the central region was found to be nonconserved. The fla sequence will facilitate the cloning of Fla proteins from other Legionella species and the study of flagella in the pathogenesis of Legionnaires' disease.

KW - Amino Acid Sequence

KW - Base Sequence

KW - Cloning, Molecular

KW - DNA, Bacterial

KW - Escherichia coli

KW - Flagellin

KW - Legionella

KW - Molecular Sequence Data

M3 - Journal article

C2 - 8562027

VL - 103

SP - 869

EP - 877

JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

SN - 0903-4641

IS - 12

ER -

ID: 40333787