The phagocytic fitness of leucopatches may impact the healing of chronic wounds
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The phagocytic fitness of leucopatches may impact the healing of chronic wounds. / Thomsen, K; Trøstrup, H; Christophersen, L.; Lundquist, R; Høiby, N; Moser, C.
In: Clinical and Experimental Immunology, Vol. 184, No. 3, 2016, p. 368-77.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The phagocytic fitness of leucopatches may impact the healing of chronic wounds
AU - Thomsen, K
AU - Trøstrup, H
AU - Christophersen, L.
AU - Lundquist, R
AU - Høiby, N
AU - Moser, C
N1 - © 2016 British Society for Immunology.
PY - 2016
Y1 - 2016
N2 - Chronic non-healing wounds are significantly bothersome to patients and can result in severe complications. In addition, they are increasing in numbers, and a challenging problem to the health-care system. Handling of chronic, non-healing wounds can be discouraging due to lack of improvement, and a recent explanation can be the involvement of biofilm infections in the pathogenesis of non-healing wounds. Therefore, new treatment alternatives to improve outcome are continuously sought-after. Autologous leucopatches are such a new, adjunctive treatment option, showing promising clinical effects. However, the beneficial effect of the patches are not understood fully, although a major contribution is believed to be from the release of stimulating growth factors from activated thrombocytes within the leucopatch. Because the leucopatches also contain substantial numbers of leucocytes, the aim of the present study was to investigate the activity of the polymorphonuclear neutrophils (PMNs) within the leucopatch. By means of burst assay, phagocytosis assay, migration assay, biofilm killing assay and fluorescence in-situ hybridization (FISH) assay we showed significant respiratory burst in PMNs, active phagocytosis and killing of Pseudomonas aeruginosa by the leucopatch. In addition, bacterial-induced migration of PMNs from the leucopatch was shown, as well as uptake of P. aeruginosa by PMNs within the leucopatch. The present study substantiated that at least part of the beneficial clinical effect in chronic wounds by leucopatches is attributed to the activity of the PMNs in the leucopatch.
AB - Chronic non-healing wounds are significantly bothersome to patients and can result in severe complications. In addition, they are increasing in numbers, and a challenging problem to the health-care system. Handling of chronic, non-healing wounds can be discouraging due to lack of improvement, and a recent explanation can be the involvement of biofilm infections in the pathogenesis of non-healing wounds. Therefore, new treatment alternatives to improve outcome are continuously sought-after. Autologous leucopatches are such a new, adjunctive treatment option, showing promising clinical effects. However, the beneficial effect of the patches are not understood fully, although a major contribution is believed to be from the release of stimulating growth factors from activated thrombocytes within the leucopatch. Because the leucopatches also contain substantial numbers of leucocytes, the aim of the present study was to investigate the activity of the polymorphonuclear neutrophils (PMNs) within the leucopatch. By means of burst assay, phagocytosis assay, migration assay, biofilm killing assay and fluorescence in-situ hybridization (FISH) assay we showed significant respiratory burst in PMNs, active phagocytosis and killing of Pseudomonas aeruginosa by the leucopatch. In addition, bacterial-induced migration of PMNs from the leucopatch was shown, as well as uptake of P. aeruginosa by PMNs within the leucopatch. The present study substantiated that at least part of the beneficial clinical effect in chronic wounds by leucopatches is attributed to the activity of the PMNs in the leucopatch.
KW - Adult
KW - Biofilms
KW - Cell Movement
KW - Female
KW - Healthy Volunteers
KW - Humans
KW - Immunologic Factors
KW - In Situ Hybridization
KW - Male
KW - Middle Aged
KW - Models, Biological
KW - Neutrophils
KW - Phagocytosis
KW - Platelet-Rich Plasma
KW - Primary Cell Culture
KW - Pseudomonas aeruginosa
KW - Reactive Oxygen Species
KW - Respiratory Burst
KW - Wound Healing
KW - Journal Article
U2 - 10.1111/cei.12773
DO - 10.1111/cei.12773
M3 - Journal article
C2 - 26830371
VL - 184
SP - 368
EP - 377
JO - Clinical and Experimental Immunology, Supplement
JF - Clinical and Experimental Immunology, Supplement
SN - 0964-2536
IS - 3
ER -
ID: 181025687