The role of individual exopolysaccharides in antibiotic tolerance of Pseudomonas aeruginosa aggregates
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The role of individual exopolysaccharides in antibiotic tolerance of Pseudomonas aeruginosa aggregates. / Liang, Ziwei; Nilsson, Martin; Kragh, Kasper Nørskov; Hedal, Ida; Alcàcer-Almansa, Júlia; Kiilerich, Rikke Overgaard; Andersen, Jens Bo; Tolker-Nielsen, Tim.
In: Frontiers in Microbiology, Vol. 14, 1187708, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The role of individual exopolysaccharides in antibiotic tolerance of Pseudomonas aeruginosa aggregates
AU - Liang, Ziwei
AU - Nilsson, Martin
AU - Kragh, Kasper Nørskov
AU - Hedal, Ida
AU - Alcàcer-Almansa, Júlia
AU - Kiilerich, Rikke Overgaard
AU - Andersen, Jens Bo
AU - Tolker-Nielsen, Tim
N1 - Publisher Copyright: Copyright © 2023 Liang, Nilsson, Kragh, Hedal, Alcàcer-Almansa, Kiilerich, Andersen and Tolker-Nielsen.
PY - 2023
Y1 - 2023
N2 - The bacterium Pseudomonas aeruginosa is involved in chronic infections of cystic fibrosis lungs and chronic wounds. In these infections the bacteria are present as aggregates suspended in host secretions. During the course of the infections there is a selection for mutants that overproduce exopolysaccharides, suggesting that the exopolysaccharides play a role in the persistence and antibiotic tolerance of the aggregated bacteria. Here, we investigated the role of individual P. aeruginosa exopolysaccharides in aggregate-associated antibiotic tolerance. We employed an aggregate-based antibiotic tolerance assay on a set of P. aeruginosa strains that were genetically engineered to over-produce a single, none, or all of the three exopolysaccharides Pel, Psl, and alginate. The antibiotic tolerance assays were conducted with the clinically relevant antibiotics tobramycin, ciprofloxacin and meropenem. Our study suggests that alginate plays a role in the tolerance of P. aeruginosa aggregates toward tobramycin and meropenem, but not ciprofloxacin. However, contrary to previous studies we did not observe a role for Psl or Pel in the tolerance of P. aeruginosa aggregates toward tobramycin, ciprofloxacin, and meropenem.
AB - The bacterium Pseudomonas aeruginosa is involved in chronic infections of cystic fibrosis lungs and chronic wounds. In these infections the bacteria are present as aggregates suspended in host secretions. During the course of the infections there is a selection for mutants that overproduce exopolysaccharides, suggesting that the exopolysaccharides play a role in the persistence and antibiotic tolerance of the aggregated bacteria. Here, we investigated the role of individual P. aeruginosa exopolysaccharides in aggregate-associated antibiotic tolerance. We employed an aggregate-based antibiotic tolerance assay on a set of P. aeruginosa strains that were genetically engineered to over-produce a single, none, or all of the three exopolysaccharides Pel, Psl, and alginate. The antibiotic tolerance assays were conducted with the clinically relevant antibiotics tobramycin, ciprofloxacin and meropenem. Our study suggests that alginate plays a role in the tolerance of P. aeruginosa aggregates toward tobramycin and meropenem, but not ciprofloxacin. However, contrary to previous studies we did not observe a role for Psl or Pel in the tolerance of P. aeruginosa aggregates toward tobramycin, ciprofloxacin, and meropenem.
KW - aggregates
KW - antibiotic tolerance
KW - biofilm
KW - extracellular matrix
KW - Pseudomonas aeruginosa
U2 - 10.3389/fmicb.2023.1187708
DO - 10.3389/fmicb.2023.1187708
M3 - Journal article
C2 - 37333638
AN - SCOPUS:85162120816
VL - 14
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
SN - 1664-302X
M1 - 1187708
ER -
ID: 358560524