Transposon Mutagenesis in Streptococcus Species

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Transposon Mutagenesis in Streptococcus Species. / Nilsson, Martin; Givskov, Michael; Tolker-Nielsen, Tim.

Microbial Transposon Mutagenesis: Protocols and Applications. Humana Press, 2019. p. 39-49 (Methods in Molecular Biology, Vol. 2016).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Nilsson, M, Givskov, M & Tolker-Nielsen, T 2019, Transposon Mutagenesis in Streptococcus Species. in Microbial Transposon Mutagenesis: Protocols and Applications. Humana Press, Methods in Molecular Biology, vol. 2016, pp. 39-49. https://doi.org/10.1007/978-1-4939-9570-7_4

APA

Nilsson, M., Givskov, M., & Tolker-Nielsen, T. (2019). Transposon Mutagenesis in Streptococcus Species. In Microbial Transposon Mutagenesis: Protocols and Applications (pp. 39-49). Humana Press. Methods in Molecular Biology Vol. 2016 https://doi.org/10.1007/978-1-4939-9570-7_4

Vancouver

Nilsson M, Givskov M, Tolker-Nielsen T. Transposon Mutagenesis in Streptococcus Species. In Microbial Transposon Mutagenesis: Protocols and Applications. Humana Press. 2019. p. 39-49. (Methods in Molecular Biology, Vol. 2016). https://doi.org/10.1007/978-1-4939-9570-7_4

Author

Nilsson, Martin ; Givskov, Michael ; Tolker-Nielsen, Tim. / Transposon Mutagenesis in Streptococcus Species. Microbial Transposon Mutagenesis: Protocols and Applications. Humana Press, 2019. pp. 39-49 (Methods in Molecular Biology, Vol. 2016).

Bibtex

@inbook{5e417d0f25954464bf1a36b0ba5d5086,
title = "Transposon Mutagenesis in Streptococcus Species",
abstract = "Mutant libraries, generated by transposons and screened for various phenotypes, have led to many important discoveries regarding gene functions in various organisms. In this chapter we describe the use of plasmid pMN100, a transposon vector constructed to perform in vivo transposition primarily in oral streptococci. Compared to in vitro transposition systems the conditional replicative features of the plasmid, and the inducible expression of the mariner Himar1 transposase, makes pMN100 particularly useful for bacterial strains showing a low transformation frequency. We outline how to transform plasmid pMN100 into Streptococcus mutans, carry out transposon mutagenesis, and determine the chromosomal location of inserted transposons. It is our prospect that the protocols can be used as guidelines for transposon mutagenesis in S. mutans as well as other species of streptococci.",
keywords = "In vivo transposon mutagenesis, Mariner, pMN100, Streptococci",
author = "Martin Nilsson and Michael Givskov and Tim Tolker-Nielsen",
year = "2019",
doi = "10.1007/978-1-4939-9570-7_4",
language = "English",
isbn = "978-1-4939-9569-1",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "39--49",
booktitle = "Microbial Transposon Mutagenesis",
address = "United States",

}

RIS

TY - CHAP

T1 - Transposon Mutagenesis in Streptococcus Species

AU - Nilsson, Martin

AU - Givskov, Michael

AU - Tolker-Nielsen, Tim

PY - 2019

Y1 - 2019

N2 - Mutant libraries, generated by transposons and screened for various phenotypes, have led to many important discoveries regarding gene functions in various organisms. In this chapter we describe the use of plasmid pMN100, a transposon vector constructed to perform in vivo transposition primarily in oral streptococci. Compared to in vitro transposition systems the conditional replicative features of the plasmid, and the inducible expression of the mariner Himar1 transposase, makes pMN100 particularly useful for bacterial strains showing a low transformation frequency. We outline how to transform plasmid pMN100 into Streptococcus mutans, carry out transposon mutagenesis, and determine the chromosomal location of inserted transposons. It is our prospect that the protocols can be used as guidelines for transposon mutagenesis in S. mutans as well as other species of streptococci.

AB - Mutant libraries, generated by transposons and screened for various phenotypes, have led to many important discoveries regarding gene functions in various organisms. In this chapter we describe the use of plasmid pMN100, a transposon vector constructed to perform in vivo transposition primarily in oral streptococci. Compared to in vitro transposition systems the conditional replicative features of the plasmid, and the inducible expression of the mariner Himar1 transposase, makes pMN100 particularly useful for bacterial strains showing a low transformation frequency. We outline how to transform plasmid pMN100 into Streptococcus mutans, carry out transposon mutagenesis, and determine the chromosomal location of inserted transposons. It is our prospect that the protocols can be used as guidelines for transposon mutagenesis in S. mutans as well as other species of streptococci.

KW - In vivo transposon mutagenesis

KW - Mariner

KW - pMN100

KW - Streptococci

U2 - 10.1007/978-1-4939-9570-7_4

DO - 10.1007/978-1-4939-9570-7_4

M3 - Book chapter

C2 - 31197707

AN - SCOPUS:85067452747

SN - 978-1-4939-9569-1

T3 - Methods in Molecular Biology

SP - 39

EP - 49

BT - Microbial Transposon Mutagenesis

PB - Humana Press

ER -

ID: 226876413