True microbiota involved in chronic lung infection of cystic fibrosis patients found by culturing and 16S rRNA gene analysis
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True microbiota involved in chronic lung infection of cystic fibrosis patients found by culturing and 16S rRNA gene analysis. / Rudkjøbing, Vibeke Børsholt; Thomsen, Trine R; Alhede, Morten; Kragh, Kasper N; Nielsen, Per H; Johansen, Ulla; Givskov, Michael; Høiby, Niels; Bjarnsholt, Thomas.
In: Journal of Clinical Microbiology, Vol. 49, No. 12, 2011, p. 4352-5.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - True microbiota involved in chronic lung infection of cystic fibrosis patients found by culturing and 16S rRNA gene analysis
AU - Rudkjøbing, Vibeke Børsholt
AU - Thomsen, Trine R
AU - Alhede, Morten
AU - Kragh, Kasper N
AU - Nielsen, Per H
AU - Johansen, Ulla
AU - Givskov, Michael
AU - Høiby, Niels
AU - Bjarnsholt, Thomas
PY - 2011
Y1 - 2011
N2 - Patients suffering from cystic fibrosis (CF) develop chronic lung infection. In this study, we investigated the microorganisms present in transplanted CF lungs (n = 5) by standard culturing and 16S rRNA gene analysis. A correspondence between culturing and the molecular methods was observed. In conclusion, standard culturing seems reliable for the identification of the dominating pathogens.
AB - Patients suffering from cystic fibrosis (CF) develop chronic lung infection. In this study, we investigated the microorganisms present in transplanted CF lungs (n = 5) by standard culturing and 16S rRNA gene analysis. A correspondence between culturing and the molecular methods was observed. In conclusion, standard culturing seems reliable for the identification of the dominating pathogens.
KW - Bacteria
KW - Biodiversity
KW - Bronchopneumonia
KW - Chronic Disease
KW - Cluster Analysis
KW - Cystic Fibrosis
KW - DNA, Bacterial
KW - DNA, Ribosomal
KW - Humans
KW - Molecular Sequence Data
KW - Phylogeny
KW - RNA, Ribosomal, 16S
KW - Sequence Analysis, DNA
U2 - 10.1128/JCM.06092-11
DO - 10.1128/JCM.06092-11
M3 - Journal article
C2 - 22012018
VL - 49
SP - 4352
EP - 4355
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
SN - 0095-1137
IS - 12
ER -
ID: 38135795