Tim Tolker-Nielsen
Professor
Bacteriology
Blegdamsvej 3B, 2200 København N
Member of:
- 2024
- Published
Role of extracellular matrix components in biofilm formation and adaptation of Pseudomonas ogarae F113 to the rhizosphere environment
Blanco-Romero, E., Garrido-Sanz, D., Durán, D., Rybtke, Morten Levin, Tolker-Nielsen, Tim, Redondo-Nieto, M., Rivilla, R. & Martín, M., 2024, In: Frontiers in Microbiology. 15, 12 p., 1341728.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Inhibition of Pseudomonas aeruginosa quorum sensing by chemical induction of the MexEF-oprN efflux pump
Kristensen, Rasmus, Andersen, Jens Bo, Rybtke, Morten Levin, Jansen, C. U., Fritz, Blaine Gabriel, Kiilerich, R. O., Uhd, J., Bjarnsholt, Thomas, Qvortrup, K., Tolker-Nielsen, Tim, Givskov, Michael & Jakobsen, Tim Holm, 2024, In: Antimicrobial Agents and Chemotherapy. 68, 2Research output: Contribution to journal › Journal article › Research › peer-review
- 2023
- Published
Colistin Enhances Rifampicin's Antimicrobial Action in Colistin-Resistant Pseudomonas aeruginosa Biofilms
Armengol, E., Kragh, K. N., Tolker-Nielsen, Tim, Sierra, J. M., Higazy, Doaa Mohamed Mohamed E, Ciofu, Oana, Viñas, M. & Høiby, Niels, 2023, In: Antimicrobial Agents and Chemotherapy. 67, 4, e0164122.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Protocol to assess metabolic activity of Pseudomonas aeruginosa by measuring heat flow using isothermal calorimetry
Beilharz, K., Kragh, K. N., Fritz, Blaine Gabriel, Kirkegaard, Julius Bier, Tolker-Nielsen, Tim, Bjarnsholt, Thomas & Lichtenberg, Mads, 2023, In: STAR Protocols. 4, 2, 102269.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
The Bep gene cluster in Burkholderia cenocepacia H111 codes for a water-insoluble exopolysaccharide essential for biofilm formation
Bellich, B., Terán, L. C., Fazli, M. M., Berti, F., Rizzo, R., Tolker-Nielsen, Tim & Cescutti, P., 2023, In: Carbohydrate Polymers. 301, 120318.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Challenges in using transcriptome data to study the c-di-GMP signaling network in Pseudomonas aeruginosa clinical isolates
Gür, M., Erdmann, J., Will, A., Liang, Z., Andersen, Jens Bo, Tolker-Nielsen, Tim & Häussler, S., 2023, In: FEMS Microbes. 4, 10 p., xtad012.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
The non-attached biofilm aggregate
Kragh, K. N., Tolker-Nielsen, Tim & Lichtenberg, Mads, 2023, In: Communications Biology . 6, 898.Research output: Contribution to journal › Review › Research › peer-review
- Published
The role of individual exopolysaccharides in antibiotic tolerance of Pseudomonas aeruginosa aggregates
Liang, Z., Nilsson, M., Kragh, K. N., Hedal, I., Alcàcer-Almansa, J., Kiilerich, R. O., Andersen, Jens Bo & Tolker-Nielsen, Tim, 2023, In: Frontiers in Microbiology. 14, 1187708.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
A genetic switch controls Pseudomonas aeruginosa surface colonization
Manner, C., Dias Teixeira, R., Saha, D., Kaczmarczyk, A., Zemp, R., Wyss, F., Jaeger, T., Laventie, B. J., Boyer, S., Malone, J. G., Qvortrup, K., Andersen, Jens Bo, Givskov, Michael, Tolker-Nielsen, Tim, Hiller, S., Drescher, K. & Jenal, U., 2023, In: Nature Microbiology. 8, p. 1520–1533Research output: Contribution to journal › Journal article › Research › peer-review
- 2022
- Published
Solid-phase synthesis and biological evaluation of piperazine-based novel bacterial topoisomerase inhibitors
Flagstad, T., Pedersen, M. T., Jakobsen, Tim Holm, Felding, J., Tolker-Nielsen, Tim, Givskov, Michael, Qvortrup, K. & Nielsen, Thomas Eiland, 2022, In: Bioorganic and Medicinal Chemistry Letters. 57, 128499.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Chemical Regulation of Pseudomonas aeruginosa Biofilm Formation as a Potential Treatment for Persistent Infections
Kristensen, Rasmus, Tolker-Nielsen, Tim & Jakobsen, Tim Holm, 2022, Antibiofilm Strategies: Current and Future Applications to Prevent, Control and Eradicate Biofilms. Springer, p. 159-179 (Springer Series on Biofilms; No. 11).Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
- Published
Transcription of the Alginate Operon in Pseudomonas aeruginosa Is Regulated by c-di-GMP
Liang, Z., Rybtke, Morten Levin, Kragh, K. N., Johnson, O., Schicketanz, Muriel Leandra, Zhang, Yong, Andersen, Jens Bo & Tolker-Nielsen, Tim, 2022, In: Microbiology Spectrum. 10, 4, 13 p., e00675-22.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Cyclic-di-GMP signaling controls metabolic activity in Pseudomonas aeruginosa
Lichtenberg, Mads, Kragh, K. N., Fritz, Blaine Gabriel, Kirkegaard, Julius Bier, Tolker-Nielsen, Tim & Bjarnsholt, Thomas, 2022, In: Cell Reports. 41, 3, p. 111515 1 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
The BDSF quorum sensing receptor RpfR regulates Bep exopolysaccharide synthesis in Burkholderia cenocepacia via interaction with the transcriptional regulator BerB
Steiner, E., Shilling, R. E., Richter, A. M., Schmid, N., Fazli, M., Kaever, V., Jenal, U., Tolker-Nielsen, Tim & Eberl, L., 2022, In: npj Biofilms and Microbiomes. 8, 93.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Effects of antibiotic treatment and phagocyte infiltration on development of Pseudomonas aeruginosa biofilm—Insights from the application of a novel PF hydrogel model in vitro and in vivo
Wu, H., Song, L., Yam, J. K. H., Plotkin, M., Wang, H., Rybtke, Morten Levin, Seliktar, D., Kofidis, T., Høiby, Niels, Tolker-Nielsen, Tim, Song, Z. & Givskov, Michael, 2022, In: Frontiers in Cellular and Infection Microbiology. 12, 826450.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Elevated c-di-GMP Levels and Expression of the Type III Secretion System Promote Corneal Infection by Pseudomonas aeruginosa
Yam, J. K. H., Aung, T. T., Chua, S. L., Cheng, Y., Kohli, G. S., Zhou, J., Constancias, F., Liu, Y., Cai, Z., Salido, M. M. S., Drautz-Moses, D. I., Rice, S. A., Schuster, S. C., Boo, Z. Z., Wu, B., Kjelleberg, S., Tolker-Nielsen, T., Lakshminarayanan, R., Beuerman, R. W., Yang, L. & 1 others, , 2022, In: Infection and Immunity. 90, 8Research output: Contribution to journal › Journal article › Research › peer-review
- 2021
- Published
Identification of small molecules that interfere with c-di-GMP signaling and induce dispersal of Pseudomonas aeruginosa biofilms
Andersen, Jens Bo, Hultqvist, Louise Dahl, Jansen, C. U., Jakobsen, Tim Holm, Nilsson, Carl Martin Peter, Rybtke, Morten Levin, Uhd, J., Fritz, Blaine Gabriel, Seifert, R., Berthelsen, J., Nielsen, Thomas Eiland, Qvortrup, K., Givskov, Michael & Tolker-Nielsen, Tim, 2021, In: npj Biofilms and Microbiomes. 7, 13 p., 59.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Induction of native c-di-GMP phosphodiesterases leads to dispersal of Pseudomonas aeruginosa biofilms
Andersen, Jens Bo, Kragh, K. N., Hultqvist, Louise Dahl, Rybtke, Morten Levin, Nilsson, Carl Martin Peter, Jakobsen, Tim Holm, Givskov, Michael & Tolker-Nielsen, Tim, 2021, In: Antimicrobial Agents and Chemotherapy. 65, 4, e02431-20.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
The biofilm of Burkholderia cenocepacia H111 contains an exopolysaccharide composed of L-rhamnose and L-mannose: Structural characterization and molecular modelling
Bellich, B., Jou, I. A., Buriola, C., Ravenscroft, N., Brady, J. W., Fazli, M., Tolker-Nielsen, Tim, Rizzo, R. & Cescutti, P., 2021, In: Carbohydrate Research. 499, 108231.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
SAR study of 4-arylazo-3,5-diamino-1: H -pyrazoles: identification of small molecules that induce dispersal of Pseudomonas aeruginosa biofilms
Jansen, C. U., Uhd, J., Andersen, Jens Bo, Hultqvist, Louise Dahl, Jakobsen, Tim Holm, Nilsson, Carl Martin Peter, Nielsen, Thomas Eiland, Givskov, Michael, Tolker-Nielsen, Tim & Qvortrup, K. M., 2021, In: RSC Medicinal Chemistry. 12, 11, p. 1868-1878 11 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Nitric-oxide-driven oxygen release in anoxic Pseudomonas aeruginosa
Lichtenberg, Mads, Line, L., Schrameyer, V., Jakobsen, Tim Holm, Rybtke, Morten Levin, Toyofuku, M., Nomura, N., Kolpen, Mette, Tolker-Nielsen, Tim, Kühl, Michael, Bjarnsholt, Thomas & Jensen, Peter Østrup, 2021, In: iScience. 24, 12, 103404.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Redox protein osar (pa0056) regulates dsbm and the oxidative stress response in pseudomonas aeruginosa
Liu, Y., Ma, Y., Ma, Z., Han, X., Qi, H., Andersen, Jens Bo, Xu, H., Tolker-Nielsen, Tim & Qiao, M., 2021, In: Antimicrobial Agents and Chemotherapy. 65, 3, e01771-20.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Immune Responses to Pseudomonas aeruginosa Biofilm Infections
Moser, Claus Ernst, Jensen, Peter Østrup, Thomsen, K., Kolpen, M., Rybtke, Morten Levin, Lauland, A. S., Trøstrup, H. & Tolker-Nielsen, Tim, 2021, In: Frontiers in Immunology. 12, 625597.Research output: Contribution to journal › Review › Research › peer-review
- Published
Carbon starvation of Pseudomonas aeruginosa biofilms selects for dispersal insensitive mutants
Nair, H. A. S., Subramoni, S., Poh, W. H., Hasnuddin, N. T. B., Tay, M., Givskov, Michael, Tolker-Nielsen, Tim, Kjelleberg, S., McDougald, D. & Rice, S. A., 2021, In: BMC Microbiology. 21, 10 p., 255.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
The Extracellular Polysaccharide Matrix of Pseudomonas aeruginosa Biofilms Is a Determinant of Polymorphonuclear Leukocyte Responses
Rybtke, Morten Levin, Jensen, Peter Østrup, Nielsen, Claus Henrik & Tolker-Nielsen, Tim, 2021, In: Infection and Immunity. 89, 1Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Biofilms can act as plasmid reserves in the absence of plasmid specific selection
Røder, Henriette Lyng, Trivedi, Urvish, Russel, J., Kragh, K. N., Herschend, J., Thalsø-Madsen, I., Tolker-Nielsen, Tim, Bjarnsholt, Thomas, Burmølle, Mette & Madsen, Jonas Stenløkke, 2021, In: npj Biofilms and Microbiomes. 7, 6 p., 78.Research output: Contribution to journal › Letter › Research › peer-review
- Published
Disruption of the Pseudomonas aeruginosa Tat system perturbs PQS-dependent quorum sensing and biofilm maturation through loss of the Rieske cytochrome bc1 sub-unit
Soh, E., Smith, F., Gimenez, M. R., Yang, L., Vejborg, R. M., Fletcher, M., Halliday, N., Bleves, S., Heeb, S., Camara, M., Givskov, Michael, Hardie, K. R., Tolker-Nielsen, Tim, Ize, B. & Williams, P., 2021, bioRxiv, 43 p.Research output: Working paper › Preprint › Research
- Published
Disruption of the Pseudomonas aeruginosa Tat system perturbs PQS-dependent quorum sensing and biofilm maturation through lack of the Rieske cytochrome bc1sub-unit
Soh, E. Y. C., Smith, F., Gimenez, M. R., Yang, L., Vejborg, R. M., Fletcher, M., Halliday, N., Bleves, S., Heeb, S., Cámara, M., Givskov, Michael, Hardie, K. R., Tolker-Nielsen, Tim, Ize, B. & Williams, P., 2021, In: PLOS Pathogens. 17, 8, 25 p., e1009425.Research output: Contribution to journal › Journal article › Research › peer-review
- 2020
- Published
Burkholderia cenocepacia h111 produces a water-insoluble exopolysaccharide in biofilm: Structural determination and molecular modelling
Bellich, B., Jou, I. A., Caterino, M., Rizzo, R., Ravenscroft, N., Fazli, M., Tolker-Nielsen, Tim, Brady, J. W. & Cescutti, P., 2020, In: International Journal of Molecular Sciences . 21, 5, 1702.Research output: Contribution to journal › Journal article › Research › peer-review
- 2019
- Published
High levels of cAMP inhibit Pseudomonas aeruginosa biofilm formation through reduction of the c-di-GMP content
Almblad, H., Rybtke, Morten Levin, Hendiani, S., Andersen, Jens Bo, Givskov, Michael & Tolker-Nielsen, Tim, Mar 2019, In: Microbiology. 165, 3, p. 324-333 10 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Tolerance and resistance of pseudomonas aeruginosabiofilms to antimicrobial agents-how P. aeruginosa Can escape antibiotics
Ciofu, Oana & Tolker-Nielsen, Tim, 2019, In: Frontiers in Microbiology. 10, MAY, 15 p., 913.Research output: Contribution to journal › Review › Research › peer-review
- Published
Sub-lethal antimicrobial photodynamic inactivation affects Pseudomonas aeruginosa PAO1 quorum sensing and cyclic di-GMP regulatory systems
Hendiani, S., Rybtke, Morten Levin, Tolker-Nielsen, Tim & Kashef, N., 2019, In: Photodiagnosis and Photodynamic Therapy. 27, p. 467-473 7 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
P. aeruginosa flow-cell biofilms are enhanced by repeated phage treatments but can be eradicated by phage-ciprofloxacin combination: —monitoring the phage–P. aeruginosa biofilms interactions
Henriksen, K., Rørbo, N., Rybtke, Morten Levin, Martinet, M. G., Tolker-Nielsen, Tim, Høiby, Niels, Middelboe, Mathias & Ciofu, Oana, 2019, In: Pathogens and Disease. 77, 2, 11 p., ftz011.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Two FtsH proteases contribute to fitness and adaptation of pseudomonas aeruginosa Clone C strains
Kamal, S. M., Rybtke, Morten Levin, Nimtz, M., Sperlein, S., Giske, C., Trček, J., Deschamps, J., Briandet, R., Dini, L., Jänsch, L., Tolker-Nielsen, Tim, Lee, C. & Römling, U., 2019, In: Frontiers in Microbiology. 10, JULY, 18 p., 1372.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Visualizing biofilm by targeting eDNA with long wavelength probe CDr15
Kwon, H. Y., Kim, J. Y., Liu, X., Lee, J. Y., Yam, J. K. H., Hultqvist, Louise Dahl, Xu, W., Rybtke, Morten Levin, Tolker-Nielsen, Tim, Heo, W., Kim, J. J., Kang, N. Y., Joo, T., Yang, L., Park, S. J., Givskov, Michael & Chang, Y. T., 2019, In: Biomaterials Science. 7, 9, p. 3594-3598 5 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Inactivation of the pgmA Gene in Streptococcus mutans Significantly Decreases Biofilm-Associated Antimicrobial Tolerance
Nilsson, Carl Martin Peter, Givskov, Michael, Twetman, Svante & Tolker-Nielsen, Tim, 2019, In: Microorganisms. 7, 9, 12 p., 310.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Oxidative stress response plays a role in antibiotic tolerance of Streptococcus mutans biofilms
Nilsson, Carl Martin Peter, Jakobsen, Tim Holm, Givskov, Michael, Twetman, Svante & Tolker-Nielsen, Tim, 2019, In: Microbiology. 165, 3, p. 334-342Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Transposon Mutagenesis in Streptococcus Species
Nilsson, Carl Martin Peter, Givskov, Michael & Tolker-Nielsen, Tim, 2019, Microbial Transposon Mutagenesis: Protocols and Applications. Humana Press, p. 39-49 11 p. (Methods in Molecular Biology, Vol. 2016).Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
- Published
Small Molecule Anti-biofilm Agents Developed on the Basis of Mechanistic Understanding of Biofilm Formation
Qvortrup, K., Hultqvist, Louise Dahl, Nilsson, Carl Martin Peter, Jakobsen, Tim Holm, Jansen, C. U., Uhd, J., Andersen, Jens Bo, Nielsen, Thomas Eiland, Givskov, Michael & Tolker-Nielsen, Tim, 2019, In: Frontiers in Chemistry. 7, 27 p., 742.Research output: Contribution to journal › Review › Research › peer-review
- Published
Key Players and Individualists of Cyclic-di-GMP Signaling in Burkholderia cenocepacia
Richter, A. M., Fazli, M., Schmid, N., Shilling, R., Suppiger, A., Givskov, Michael, Eberl, L. & Tolker-Nielsen, Tim, 2019, In: Frontiers in Microbiology. 9, 15 p., 3286.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Increased Intracellular Cyclic di-AMP Levels Sensitize Streptococcus gallolyticus subsp. gallolyticus to Osmotic Stress and Reduce Biofilm Formation and Adherence on Intestinal Cells
Teh, W. K., Dramsi, S., Tolker-Nielsen, Tim, Yang, L. & Givskov, Michael, 2019, In: Journal of Bacteriology. 201, 6, p. 1-16 16 p.Research output: Contribution to journal › Journal article › Research › peer-review
- 2018
- Published
Pseudomonas aeruginosa rhamnolipid induces fibrillation of human α‐synuclein and modulates its effect on biofilm formation
Andersen, K. K., Vad, B. S., Kjaer, L., Tolker-Nielsen, Tim, Christiansen, G. & Otzen, D. E., May 2018, In: FEBS Letters. 592, 9, p. 1484-1496 13 p.Research output: Contribution to journal › Letter › Research › peer-review
- Published
Inoculation method could impact the outcome of microbiological experiments
Kragh, K. N., Alhede, M., Rybtke, Morten Levin, Stavnsbjerg, Camilla, Tolker-Nielsen, Tim, Whiteley, M. & Bjarnsholt, Thomas, 2018, In: Applied and Environmental Microbiology. 84, 5, 14 p., e02264-17.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
CDy14: A novel biofilm probe targeting exopolysaccharide Psl
Kwon, H. Y., Kim, J. Y., Lee, J. Y., Yam, J. K. H., Hultqvist, Louise Dahl, Xu, W., Rybtke, Morten Levin, Tolker-Nielsen, Tim, Kim, J. J., Kang, N. Y., Yang, L., Park, S. J., Givskov, Michael & Chang, Y. T., 2018, In: Chemical Communications. 54, 84, p. 11865-11868 4 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
A transposon mutant library of Bacillus cereus ATCC 10987 reveals novel genes required for biofilm formation and implicates motility as an important factor for pellicle-biofilm formation
Okshevsky, M., Louw, M. G., Lamela, E. O., Nilsson, Carl Martin Peter, Tolker-Nielsen, Tim & Meyer, R. L., 2018, In: MicrobiologyOpen. 7, 2, 17 p., e00552.Research output: Contribution to journal › Journal article › Research › peer-review
- 2017
- Published
Selective proteomic analysis of antibiotic-tolerant cellular subpopulations in pseudomonas aeruginosa biofilms
Babin, B. M., Atangcho, L., van Eldijk, M. B., Sweredoski, M. J., Moradian, A., Hess, S., Tolker-Nielsen, Tim, Newman, D. K. & Tirrell, D. A., 2017, In: mBio. 8, 5, 16 p., e01593-17.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Reduced intracellular c-di-GMP content increases expression of quorum sensing-regulated genes in Pseudomonas aeruginosa
Chua, S. L., Liu, Y., Li, Y., Ting, H. J., Kohli, G. S., Cai, Z., Suwanchaikasem, P., Goh, K. K. K., Ng, S. P., Tolker-Nielsen, Tim, Yang, L. & Givskov, Michael, Oct 2017, In: Frontiers in Cellular and Infection Microbiology. 7, 8 p., 451.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB
Fazli, M., Rybtke, Morten Levin, Steiner, E., Weidel, E., Berthelsen, J., Groizeleau, J. D. D., Bin, W., Zhi, B. Z., Yaming, Z., Kaever, V., Givskov, Michael, Hartmann, R. W., Eberl, L. & Tolker-Nielsen, Tim, 2017, In: MicrobiologyOpen. 6, 4, 13 p., e00480.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Importance of the exopolysaccharide matrix in antimicrobial tolerance of Pseudomonas aeruginosa aggregates
Goltermann, Lise & Tolker-Nielsen, Tim, Apr 2017, In: Antimicrobial Agents and Chemotherapy. 61, 4, 7 p., e02696.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
High-throughput screening for compounds that modulate the cellular c-di-GMP level in bacteria
Groizeleau, J., Andersen, Jens Bo, Givskov, Michael, Berthelsen, J. & Tolker-Nielsen, Tim, Jan 2017, c-di-GMP Signaling: Methods and Protocols. Sauer, K. (ed.). Humana Press, Vol. 1657. p. 455-470 16 p. (Methods in Molecular Biology, Vol. 1657).Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
ID: 8656938
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Colony morphology and transcriptome profiling of Pseudomonas putida KT2440 and its mutants deficient in alginate or all EPS synthesis under controlled matric potentials
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C-di-GMP regulates Pseudomonas aeruginosa stress response to tellurite during both planktonic and biofilm modes of growth
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Bacterial Biofilm Control by Perturbation of Bacterial Signaling Processes
Research output: Contribution to journal › Review › Research › peer-review
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