Tim Tolker-Nielsen
Professor
Bacteriology
Blegdamsvej 3B, 2200 København N
Member of:
- Published
The catabolite repression control protein Crc plays a role in the development of antimicrobial-tolerant subpopulations in Pseudomonas aeruginosa biofilms
Zhang, L., Chiang, W., Gao, Q., Givskov, Michael, Tolker-Nielsen, Tim, Yang, L. & Zhang, G., Dec 2012, In: Microbiology. 158, 12, p. 3014-3019 6 p.Research output: Contribution to journal › Journal article › Research › peer-review
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Functional bacterial amyloid increases Pseudomonas biofilm hydrophobicity and stiffness
Zeng, G., Vad, B. S., Dueholm, M. S., Christiansen, G., Nilsson, Carl Martin Peter, Tolker-Nielsen, Tim, Nielsen, P. H., Meyer, R. L. & Otzen, D. E., 2015, In: Frontiers in Microbiology. 6, 14 p., 1099.Research output: Contribution to journal › Journal article › Research › peer-review
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Computer-aided identification of recognized drugs as Pseudomonas aeruginosa quorum-sensing inhibitors
Yang, L., Rybtke, Morten Levin, Jakobsen, Tim Holm, Hentzer, M., Bjarnsholt, Thomas, Givskov, Michael & Tolker-Nielsen, Tim, 2009, In: Antimicrobial Agents and Chemotherapy. 53, 6, p. 2432-43 12 p.Research output: Contribution to journal › Journal article › Research › peer-review
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Pyoverdine and PQS mediated subpopulation interactions involved in Pseudomonas aeruginosa biofilm formation
Yang, L., Nilsson, Carl Martin Peter, Gjermansen, M., Givskov, Michael & Tolker-Nielsen, Tim, 2009, In: Molecular Microbiology. 74, 6, p. 1380-92 12 p.Research output: Contribution to journal › Journal article › Research › peer-review
Effects of iron on DNA release and biofilm development by Pseudomonas aeruginosa.
Yang, L., Barken, K. B., Skindersoe, M. E., Christensen, A. B., Givskov, M. & Tolker-Nielsen, Tim, 2007, In: Microbiology. 153, Pt 5, p. 1318-28 10 p.Research output: Contribution to journal › Journal article › Research › peer-review
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Pattern differentiation in co-culture biofilms formed by Staphylococcus aureus and Pseudomonas aeruginosa
Yang, L., Liu, Y., Markussen, T., Høiby, Niels, Tolker-Nielsen, Tim & Molin, S., 1 Aug 2011, In: F E M S Immunology and Medical Microbiology. 62, 3, p. 339-47 9 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Elevated c-di-GMP Levels and Expression of the Type III Secretion System Promote Corneal Infection by Pseudomonas aeruginosa
Yam, J. K. H., Aung, T. T., Chua, S. L., Cheng, Y., Kohli, G. S., Zhou, J., Constancias, F., Liu, Y., Cai, Z., Salido, M. M. S., Drautz-Moses, D. I., Rice, S. A., Schuster, S. C., Boo, Z. Z., Wu, B., Kjelleberg, S., Tolker-Nielsen, T., Lakshminarayanan, R., Beuerman, R. W., Yang, L. & 1 others, , 2022, In: Infection and Immunity. 90, 8Research output: Contribution to journal › Journal article › Research › peer-review
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Effects of antibiotic treatment and phagocyte infiltration on development of Pseudomonas aeruginosa biofilm—Insights from the application of a novel PF hydrogel model in vitro and in vivo
Wu, H., Song, L., Yam, J. K. H., Plotkin, M., Wang, H., Rybtke, Morten Levin, Seliktar, D., Kofidis, T., Høiby, Niels, Tolker-Nielsen, Tim, Song, Z. & Givskov, Michael, 2022, In: Frontiers in Cellular and Infection Microbiology. 12, 826450.Research output: Contribution to journal › Journal article › Research › peer-review
Pseudomonas aeruginosa fimL regulates multiple virulence functions by intersecting with Vfr-modulated pathways.
Whitchurch, C. B., Beatson, S. A., Comolli, J. C., Jakobsen, T., Sargent, J. L., Bertrand, J. J., West, J., Klausen, M., Waite, L. L., Kang, P. J., Tolker-Nielsen, Tim, Mattick, J. S. & Engel, J. N., 2005, In: Molecular Microbiology. 55, 5, p. 1357-78 21 p.Research output: Contribution to journal › Journal article › Research › peer-review
Extracellular DNA required for bacterial biofilm formation.
Whitchurch, C. B., Tolker-Nielsen, Tim, Ragas, P. C. & Mattick, J. S., 2002, In: Science. 295, 5559, p. 1487Research output: Contribution to journal › Journal article › Research › peer-review
Cell death in Pseudomonas aeruginosa biofilm development.
Webb, J. S., Thompson, L. S., James, S., Charlton, T., Tolker-Nielsen, Tim, Koch, B., Givskov, M. & Kjelleberg, S., 2003, In: Journal of Bacteriology. 185, 15, p. 4585-92 7 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Engineering PQS biosynthesis pathway for enhancement of bioelectricity production in Pseudomonas aeruginosa microbial fuel cells
Wang, V. B., Chua, S., Cao, B., Seviour, T., Nesatyy, V. J., Marsili, E., Kjelleberg, S., Givskov, Michael, Tolker-Nielsen, Tim, Song, H., Loo, J. S. C. & Yang, L., 2013, In: PLOS ONE. 8, 5, p. e63129 7 p., e63129.Research output: Contribution to journal › Journal article › Research › peer-review
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Pseudomonas aeruginosa LysR PA4203 regulator NmoR acts as a repressor of the PA4202 nmoA gene, encoding a nitronate monooxygenase
Vercammen, K., Wei, Q., Charlier, D., Dötsch, A., Haüssler, S., Schulz, S., Salvi, F., Gadda, G., Spain, J., Rybtke, Morten Levin, Tolker-Nielsen, Tim, Dingemans, J., Ye, L. & Cornelis, P., Mar 2015, In: Journal of Bacteriology. 197, 6, p. 1026-39 14 p.Research output: Contribution to journal › Journal article › Research › peer-review
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Inactivation of the rhlA gene in Pseudomonas aeruginosa prevents rhamnolipid production, disabling the protection against polymorphonuclear leukocytes
Van Gennip, M., Hultqvist, Louise Dahl, Alhede, M., Phipps, R., Jensen, Peter Østrup, Christophersen, L., Pamp, S. J., Moser, C., Mikkelsen, P. J., Koh, A. Y., Tolker-Nielsen, Tim, Pier, G. B., Høiby, Niels, Givskov, Michael & Bjarnsholt, Thomas, 1 Jul 2009, In: Acta Pathologica Microbiologica et Immunologica Scandinavica. 117, 7, p. 537-46 10 p.Research output: Contribution to journal › Journal article › Research › peer-review
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The Pseudomonas aeruginosa Type III Translocon Is Required for Biofilm Formation at the Epithelial Barrier
Tran, C. S., Rangel, S. M., Almblad, H., Kierbel, A., Givskov, Michael, Tolker-Nielsen, Tim, Hauser, A. R. & Engel, J. N., Nov 2014, In: P L o S Pathogens. 10, 11, p. 1-11 12 p., e1004479.Research output: Contribution to journal › Journal article › Research › peer-review
Non-genetic population heterogeneity studied by in situ polymerase chain reaction.
Tolker-Nielsen, Tim, Holmstrøm, K., Boe, L. & Molin, S., 1998, In: Molecular Microbiology. 27, 6, p. 1099-105 6 p.Research output: Contribution to journal › Journal article › Research › peer-review
Visualization of specific gene expression in individual Salmonella typhimurium cells by in situ PCR.
Tolker-Nielsen, Tim, Holmstrøm, K. & Molin, S., 1997, In: Applied and Environmental Microbiology. 63, 11, p. 4196-203 7 p.Research output: Contribution to journal › Journal article › Research › peer-review
The biofilm lifestyle of pseudomonads.
Tolker-Nielsen, Tim & Molin, S., 2004, Pseudomonas. Ramos, J. (ed.). Kluwer Academic Publishers, p. 547-571Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research
Role of ribosome degradation in the death of heat-stressed Salmonella typhimurium.
Tolker-Nielsen, Tim & Molin, S., 1996, In: FEMS Microbiology Letters. 142, 2-3, p. 155-60 5 p.Research output: Contribution to journal › Journal article › Research › peer-review
Effects of stress treatments on the detection of Salmonella typhimurium by in situ hybridization.
Tolker-Nielsen, Tim, Larsen, M. H., Kyed, H. & Molin, S., 1997, In: International Journal of Food Microbiology. 35, 3, p. 251-8 7 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Pseudomonas aeruginosa biofilm infections: From molecular biofilm biology to new treatment possibilities
Tolker-Nielsen, Tim, Dec 2014, 51 p. (APMIS Supplementum).Research output: Book/Report › Doctoral thesis › Research
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Biofilm development
Tolker-Nielsen, Tim, 2015, Microbial biofilms. Ghannoum, M., Parsek, M., Whiteley, M. & P. M. (eds.). 2 ed. Washington DC: ASM Press, p. 51-66 16 p.Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Spatial Organization of Microbial Biofilm Communities.
Tolker-Nielsen, Tim & Molin, S., 2000, In: Microbial Ecology. 40, 2, p. 75-84 9 p.Research output: Contribution to journal › Journal article › Research › peer-review
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Biofilm development
Tolker-Nielsen, Tim, 1 Jan 2015, Microbial Biofilms. Wiley-Interscience, p. 51-66 16 p.Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Biofilm development by pseudomonads.
Tolker-Nielsen, Tim & Molin, S., 2004, Environmental Biotechnology. Verstraete, W. (ed.). CRC Press/Balkema, p. 27-32Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research
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Methods for studying biofilm formation: flow cells and confocal laser scanning microscopy
Tolker-Nielsen, Tim & Sternberg, C., 2014, In: Methods in Molecular Biology. 1149, p. 615-29 15 p.Research output: Contribution to journal › Journal article › Research › peer-review
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Biofilm Development
Tolker-Nielsen, Tim, Mar 2015, In: Microbiology Spectrum. 3, 2, 12 p., MB-0001-2014.Research output: Contribution to journal › Journal article › Research › peer-review
Assessment of flhDC mRNA levels in Serratia liquefaciens swarm cells.
Tolker-Nielsen, Tim, Christensen, A. B., Holmstrøm, K., Eberl, L., Rasmussen, T. B., Sternberg, C., Heydorn, A., Molin, S. & Givskov, M., 2000, In: Journal of Bacteriology. 182, 10, p. 2680-6 6 p.Research output: Contribution to journal › Journal article › Research › peer-review
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Growing and analyzing biofilms in flow chambers
Tolker-Nielsen, Tim & Sternberg, C., 2011, In: Current Protocols in Microbiology. 21, p. 1B.2.1-1B.2.17Research output: Contribution to journal › Journal article › Research › peer-review
A statistical analysis of the formation of plasmid-free cells in populations of Escherichia coli.
Tolker-Nielsen, Tim & Boe, L., 1994, In: Journal of Bacteriology. 176, 14, p. 4306-10 4 p.Research output: Contribution to journal › Journal article › Research › peer-review
Development and dynamics of Pseudomonas sp. biofilms.
Tolker-Nielsen, Tim, Brinch, U. C., Ragas, P. C., Andersen, J. B., Jacobsen, C. S. & Molin, S., 2000, In: Journal of Bacteriology. 182, 22, p. 6482-9 7 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Extracellular DNA and F-actin as targets in antibiofilm cystic fibrosis therapy
Tolker-Nielsen, Tim & Høiby, Niels, 2009, In: Future Microbiology. 4, p. 645-7 3 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
The bactericidal activity of β-lactam antibiotics is increased by metabolizable sugar species
Thorsing, M., Bentin, Thomas, Givskov, Michael, Tolker-Nielsen, Tim & Goltermann, Lise, Oct 2015, In: Microbiology. 161, 10, p. 1999-2007 9 p.Research output: Contribution to journal › Journal article › Research › peer-review
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Increased Intracellular Cyclic di-AMP Levels Sensitize Streptococcus gallolyticus subsp. gallolyticus to Osmotic Stress and Reduce Biofilm Formation and Adherence on Intestinal Cells
Teh, W. K., Dramsi, S., Tolker-Nielsen, Tim, Yang, L. & Givskov, Michael, 2019, In: Journal of Bacteriology. 201, 6, p. 1-16 16 p.Research output: Contribution to journal › Journal article › Research › peer-review
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Draft Genome Sequence of the Model Naphthalene-Utilizing Organism Pseudomonas putida OUS82
Tay, M., Roizman, D., Cohen, Y., Tolker-Nielsen, Tim, Givskov, Michael & Yang, L., 2014, In: Genome Announcements. 2, 1, p. 1 1 p., e01161-13.Research output: Contribution to journal › Journal article › Research › peer-review
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Comparative systems biology analysis to study the mode of action of the isothiocyanate compound Iberin on Pseudomonas aeruginosa
Tan, S. Y., Liu, Y., Chua, S. L., Vejborg, R. M., Jakobsen, Tim Holm, Chew, S. C., Li, Y., Nielsen, T. E., Tolker-Nielsen, Tim, Yang, L. & Givskov, Michael, Nov 2014, In: Antimicrobial Agents and Chemotherapy. 58, 11, p. 6648-59 12 p.Research output: Contribution to journal › Journal article › Research › peer-review
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Cranberry (Vaccinium macrocarpon) oligosaccharides decrease biofilm formation by uropathogenic Escherichia coli
Sun, J., Marais, J. P. J., Khoo, C., LaPlante, K., Vejborg, R. M., Givskov, Michael, Tolker-Nielsen, Tim, Seeram, N. P. & Rowley, D. C., Aug 2015, In: Journal of Functional Foods. 17, p. 235-242 8 p.Research output: Contribution to journal › Letter › Research › peer-review
Growing and analyzing biofilms in flow cells.
Sternberg, C. & Tolker-Nielsen, Tim, 2006, In: Current Protocols in Microbiology. Chapter 1, p. Unit 1B.2Research output: Contribution to journal › Journal article › Research › peer-review
- Published
The BDSF quorum sensing receptor RpfR regulates Bep exopolysaccharide synthesis in Burkholderia cenocepacia via interaction with the transcriptional regulator BerB
Steiner, E., Shilling, R. E., Richter, A. M., Schmid, N., Fazli, M., Kaever, V., Jenal, U., Tolker-Nielsen, Tim & Eberl, L., 2022, In: npj Biofilms and Microbiomes. 8, 93.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Synthesis and biological evaluation of triazole-containing N-acyl homoserine lactones as quorum sensing modulators
Stacy, D. M., Le Quement, S. T., Hansen, C., Clausen, J. W., Tolker-Nielsen, Tim, Brummond, J. W., Givskov, Michael, Nielsen, T. E. & Blackwell, H. E., 2013, In: Organic & Biomolecular Chemistry. 11, 6, p. 938-54 17 p.Research output: Contribution to journal › Journal article › Research › peer-review
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Disruption of the Pseudomonas aeruginosa Tat system perturbs PQS-dependent quorum sensing and biofilm maturation through lack of the Rieske cytochrome bc1sub-unit
Soh, E. Y. C., Smith, F., Gimenez, M. R., Yang, L., Vejborg, R. M., Fletcher, M., Halliday, N., Bleves, S., Heeb, S., Cámara, M., Givskov, Michael, Hardie, K. R., Tolker-Nielsen, Tim, Ize, B. & Williams, P., 2021, In: PLOS Pathogens. 17, 8, 25 p., e1009425.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Disruption of the Pseudomonas aeruginosa Tat system perturbs PQS-dependent quorum sensing and biofilm maturation through loss of the Rieske cytochrome bc1 sub-unit
Soh, E., Smith, F., Gimenez, M. R., Yang, L., Vejborg, R. M., Fletcher, M., Halliday, N., Bleves, S., Heeb, S., Camara, M., Givskov, Michael, Hardie, K. R., Tolker-Nielsen, Tim, Ize, B. & Williams, P., 2021, bioRxiv, 43 p.Research output: Working paper › Preprint
- Published
Identification of LasR ligands through a virtual screening approach
Skovstrup, S., Le Quement, S. T., Hansen, T., Jakobsen, Tim Holm, Harmsen, M., Tolker-Nielsen, Tim, Nielsen, T. E., Givskov, Michael & Taboureau, O., 2013, In: ChemMedChem. 8, 1, p. 157-63 7 p.Research output: Contribution to journal › Journal article › Research › peer-review
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Effects of antibiotics on quorum sensing in Pseudomonas aeruginosa
Skindersoe, M. E., Alhede, M., Phipps, R., Yang, L., Jensen, P. O., Rasmussen, T. B., Bjarnsholt, Thomas, Tolker-Nielsen, Tim, Høiby, Niels & Givskov, Michael, 1 Oct 2008, In: Antimicrobial Agents and Chemotherapy. 52, 10, p. 3648-63 16 p.Research output: Contribution to journal › Journal article › Research › peer-review
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The contribution of cell-cell signaling and motility to bacterial biofilm formation
Shrout, J. D., Tolker-Nielsen, Tim, Givskov, Michael & Parsek, M. R., May 2011, In: M R S Bulletin. 36, 5, p. 367-373 7 p.Research output: Contribution to journal › Journal article › Research › peer-review
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Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions
Schwarz, S., West, T. E., Boyer, F., Chiang, W., Carl, M. A., Hood, R. D., Rohmer, L., Tolker-Nielsen, Tim, Skerrett, S. J. & Mougous, J. D., 2010, In: PLoS Pathogens. 6, 8Research output: Contribution to journal › Journal article › Research › peer-review
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High intracellular c-di-GMP levels antagonize quorum sensing and virulence gene expression in Burkholderia cenocepacia H111
Schmid, N., Suppiger, A., Steiner, E., Pessi, G., Kaever, V., Fazli, M., Tolker-Nielsen, Tim, Jenal, U. & Eberl, L., 1 May 2017, In: Microbiology. 163, 5, p. 754-764 11 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Biofilms can act as plasmid reserves in the absence of plasmid specific selection
Røder, Henriette Lyng, Trivedi, Urvish, Russel, J., Kragh, K. N., Herschend, J., Thalsø-Madsen, I., Tolker-Nielsen, Tim, Bjarnsholt, Thomas, Burmølle, Mette & Madsen, Jonas Stenløkke, 2021, In: npj Biofilms and Microbiomes. 7, 6 p., 78.Research output: Contribution to journal › Letter › Research › peer-review
- Published
The implication of Pseudomonas aeruginosa biofilms in infections
Rybtke, Morten Levin, Jensen, Peter Østrup, Høiby, Niels, Givskov, Michael, Tolker-Nielsen, Tim & Bjarnsholt, Thomas, 2011, In: Inflammation & Allergy - Drug Targets. 10, 2, p. 141-57 17 p.Research output: Contribution to journal › Journal article › Research › peer-review
- Published
Fluorescence-based reporter for gauging cyclic di-GMP Levels in Pseudomonas aeruginosa.
Rybtke, Morten Levin, Borlee, B. R., Murakami, K., Irie, Y., Hentzer, M., Nielsen, T. E., Givskov, Michael, Parsek, M. R. & Tolker-Nielsen, Tim, 2012, In: Applied and Environmental Microbiology. 78, 15, p. 5060-5069 10 p.Research output: Contribution to journal › Journal article › Research › peer-review
ID: 8656938
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Colony morphology and transcriptome profiling of Pseudomonas putida KT2440 and its mutants deficient in alginate or all EPS synthesis under controlled matric potentials
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C-di-GMP regulates Pseudomonas aeruginosa stress response to tellurite during both planktonic and biofilm modes of growth
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Bacterial Biofilm Control by Perturbation of Bacterial Signaling Processes
Research output: Contribution to journal › Review › Research › peer-review
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