Assessment of flhDC mRNA levels in Serratia liquefaciens swarm cells.
Research output: Contribution to journal › Journal article › Research › peer-review
We reported previously that artificial overexpression of the flhDC operon in liquid-grown Serratia liquefaciens resulted in the formation of filamentous, multinucleated, and hyperflagellated cells that were indistinguishable from surface-induced swarm cells (L. Eberl, G. Christiansen, S. Molin, and M. Givskov, J. Bacteriol. 178:554-559, 1996). In the present report we show by means of reporter gene measurements, Northern analysis, and in situ reverse transcription-PCR that the amount of flhDC mRNA in surface-grown swarm cells does not exceed the maximum level found in nondifferentiated, vegetative cells. This suggests that surface-induced S. liquefaciens swarm cell differentiation, although dependent on flhDC gene expression, does not occur through elevated flhDC mRNA levels.
Original language | English |
---|---|
Journal | Journal of Bacteriology |
Volume | 182 |
Issue number | 10 |
Pages (from-to) | 2680-6 |
Number of pages | 6 |
ISSN | 0021-9193 |
Publication status | Published - 2000 |
Externally published | Yes |
Bibliographical note
Keywords: Bacterial Proteins; DNA-Binding Proteins; Escherichia coli Proteins; Gene Expression; Gene Expression Regulation, Bacterial; Isopropyl Thiogalactoside; Operon; RNA, Bacterial; RNA, Messenger; Recombinant Fusion Proteins; Serratia; Trans-Activators
ID: 8780498