Assessment of flhDC mRNA levels in Serratia liquefaciens swarm cells.

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Assessment of flhDC mRNA levels in Serratia liquefaciens swarm cells. / Tolker-Nielsen, Tim; Christensen, A B; Holmstrøm, K; Eberl, L; Rasmussen, T B; Sternberg, C; Heydorn, A; Molin, S; Givskov, Michael.

In: Journal of Bacteriology, Vol. 182, No. 10, 2000, p. 2680-6.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Tolker-Nielsen, T, Christensen, AB, Holmstrøm, K, Eberl, L, Rasmussen, TB, Sternberg, C, Heydorn, A, Molin, S & Givskov, M 2000, 'Assessment of flhDC mRNA levels in Serratia liquefaciens swarm cells.', Journal of Bacteriology, vol. 182, no. 10, pp. 2680-6.

APA

Tolker-Nielsen, T., Christensen, A. B., Holmstrøm, K., Eberl, L., Rasmussen, T. B., Sternberg, C., Heydorn, A., Molin, S., & Givskov, M. (2000). Assessment of flhDC mRNA levels in Serratia liquefaciens swarm cells. Journal of Bacteriology, 182(10), 2680-6.

Vancouver

Tolker-Nielsen T, Christensen AB, Holmstrøm K, Eberl L, Rasmussen TB, Sternberg C et al. Assessment of flhDC mRNA levels in Serratia liquefaciens swarm cells. Journal of Bacteriology. 2000;182(10):2680-6.

Author

Tolker-Nielsen, Tim ; Christensen, A B ; Holmstrøm, K ; Eberl, L ; Rasmussen, T B ; Sternberg, C ; Heydorn, A ; Molin, S ; Givskov, Michael. / Assessment of flhDC mRNA levels in Serratia liquefaciens swarm cells. In: Journal of Bacteriology. 2000 ; Vol. 182, No. 10. pp. 2680-6.

Bibtex

@article{020e5d90bd4211dd8e02000ea68e967b,
title = "Assessment of flhDC mRNA levels in Serratia liquefaciens swarm cells.",
abstract = "We reported previously that artificial overexpression of the flhDC operon in liquid-grown Serratia liquefaciens resulted in the formation of filamentous, multinucleated, and hyperflagellated cells that were indistinguishable from surface-induced swarm cells (L. Eberl, G. Christiansen, S. Molin, and M. Givskov, J. Bacteriol. 178:554-559, 1996). In the present report we show by means of reporter gene measurements, Northern analysis, and in situ reverse transcription-PCR that the amount of flhDC mRNA in surface-grown swarm cells does not exceed the maximum level found in nondifferentiated, vegetative cells. This suggests that surface-induced S. liquefaciens swarm cell differentiation, although dependent on flhDC gene expression, does not occur through elevated flhDC mRNA levels.",
author = "Tim Tolker-Nielsen and Christensen, {A B} and K Holmstr{\o}m and L Eberl and Rasmussen, {T B} and C Sternberg and A Heydorn and S Molin and Michael Givskov",
note = "Keywords: Bacterial Proteins; DNA-Binding Proteins; Escherichia coli Proteins; Gene Expression; Gene Expression Regulation, Bacterial; Isopropyl Thiogalactoside; Operon; RNA, Bacterial; RNA, Messenger; Recombinant Fusion Proteins; Serratia; Trans-Activators",
year = "2000",
language = "English",
volume = "182",
pages = "2680--6",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "10",

}

RIS

TY - JOUR

T1 - Assessment of flhDC mRNA levels in Serratia liquefaciens swarm cells.

AU - Tolker-Nielsen, Tim

AU - Christensen, A B

AU - Holmstrøm, K

AU - Eberl, L

AU - Rasmussen, T B

AU - Sternberg, C

AU - Heydorn, A

AU - Molin, S

AU - Givskov, Michael

N1 - Keywords: Bacterial Proteins; DNA-Binding Proteins; Escherichia coli Proteins; Gene Expression; Gene Expression Regulation, Bacterial; Isopropyl Thiogalactoside; Operon; RNA, Bacterial; RNA, Messenger; Recombinant Fusion Proteins; Serratia; Trans-Activators

PY - 2000

Y1 - 2000

N2 - We reported previously that artificial overexpression of the flhDC operon in liquid-grown Serratia liquefaciens resulted in the formation of filamentous, multinucleated, and hyperflagellated cells that were indistinguishable from surface-induced swarm cells (L. Eberl, G. Christiansen, S. Molin, and M. Givskov, J. Bacteriol. 178:554-559, 1996). In the present report we show by means of reporter gene measurements, Northern analysis, and in situ reverse transcription-PCR that the amount of flhDC mRNA in surface-grown swarm cells does not exceed the maximum level found in nondifferentiated, vegetative cells. This suggests that surface-induced S. liquefaciens swarm cell differentiation, although dependent on flhDC gene expression, does not occur through elevated flhDC mRNA levels.

AB - We reported previously that artificial overexpression of the flhDC operon in liquid-grown Serratia liquefaciens resulted in the formation of filamentous, multinucleated, and hyperflagellated cells that were indistinguishable from surface-induced swarm cells (L. Eberl, G. Christiansen, S. Molin, and M. Givskov, J. Bacteriol. 178:554-559, 1996). In the present report we show by means of reporter gene measurements, Northern analysis, and in situ reverse transcription-PCR that the amount of flhDC mRNA in surface-grown swarm cells does not exceed the maximum level found in nondifferentiated, vegetative cells. This suggests that surface-induced S. liquefaciens swarm cell differentiation, although dependent on flhDC gene expression, does not occur through elevated flhDC mRNA levels.

M3 - Journal article

C2 - 10781533

VL - 182

SP - 2680

EP - 2686

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 10

ER -

ID: 8780498