Comparison of UVB and UVC irradiation disinfection efficacies on Pseudomonas Aeruginosa (P. aeruginosa) biofilm
Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review
Disinfection routines are important in all clinical applications. The uprising problem of antibiotic resistance has driven major research efforts towards alternative disinfection approaches, involving light-based solutions. Pseudomonas aeruginosa (P. aeruginosa) is a common bacterium that can cause skin, soft tissue, lungs, kidney and urinary tract infections. Moreover, it can be found on and in medical equipment causing often cross infections in hospitals. The objective of this study was to test the efficiency, of two different light-based disinfection treatments, namely UVB and UVC irradiation, on P. aeruginosa biofilms at different growth stages. In our experiments a new type of UV light emitting diodes (LEDs) were used to deliver UV irradiation on the biofilms, in the UVB (296nm) and UVC (266nm) region. The killing rate was studied as a function of dose for 24h grown biofilms. The dose was ramped from 72J/m2 to 10000J/m2. It was shown that UVB irradiation was more effective than UVC irradiation in inactivating P. aeruginosa biofilms. No colony forming units (CFU) were observed for the UVB treated biofilms when the dose was 10000 J/m2 (CFU in control sample: 7.5 x 104). UVB irradiation at a dose of 20000J/m2 on mature biofilms (72h grown) resulted in a 3.9 log killing efficacy. The fact that the wavelength of 296nm exists in daylight and has such disinfection ability on biofilms gives new perspectives for applications within disinfection at hospitals.
Original language | English |
---|---|
Title of host publication | Biophotonics : Photonic Solutions for Better Health Care V |
Number of pages | 7 |
Volume | 9887 |
Publisher | SPIE - International Society for Optical Engineering |
Publication date | 2016 |
Article number | 988730 |
ISBN (Electronic) | 9781510601321 |
DOIs | |
Publication status | Published - 2016 |
Event | Biophotonics: Photonic Solutions for Better Health Care V - Brussels, Belgium Duration: 4 Apr 2016 → 7 Apr 2016 |
Conference
Conference | Biophotonics: Photonic Solutions for Better Health Care V |
---|---|
Land | Belgium |
By | Brussels |
Periode | 04/04/2016 → 07/04/2016 |
Sponsor | Brussels Photonics Team (B-PHOT), Research Foundation Flanders, The Society of Photo-Optical Instrumentation Engineers (SPIE), Visit Brussels |
Series | Proceedings of S P I E - International Society for Optical Engineering |
---|---|
Volume | 9887 |
ISSN | 0277-786X |
- Disinfection, Light emitting diodes (LEDs), Pseudomonas aeruginosa (P. aeruginosa), Sterilization, Ultraviolet light, UVB, UVC
Research areas
ID: 168855551