Endolysin Inhibits Skin Colonization by Patient-Derived Staphylococcus Aureus and Malignant T-Cell Activation in Cutaneous T-Cell Lymphoma

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Endolysin Inhibits Skin Colonization by Patient-Derived Staphylococcus Aureus and Malignant T-Cell Activation in Cutaneous T-Cell Lymphoma. / Pallesen, Emil M.H.; Gluud, Maria; Vadivel, Chella Krishna; Buus, Terkild B.; de Rooij, Bob; Zeng, Ziao; Ahmad, Sana; Willerslev-Olsen, Andreas; Röhrig, Christian; Kamstrup, Maria R.; Bay, Lene; Lindahl, Lise; Krejsgaard, Thorbjørn; Geisler, Carsten; Bonefeld, Charlotte M.; Iversen, Lars; Woetmann, Anders; Koralov, Sergei B.; Bjarnsholt, Thomas; Frieling, Johan; Schmelcher, Mathias; Ødum, Niels.

In: Journal of Investigative Dermatology, Vol. 143, No. 9, 2023, p. 1757-1769.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Pallesen, EMH, Gluud, M, Vadivel, CK, Buus, TB, de Rooij, B, Zeng, Z, Ahmad, S, Willerslev-Olsen, A, Röhrig, C, Kamstrup, MR, Bay, L, Lindahl, L, Krejsgaard, T, Geisler, C, Bonefeld, CM, Iversen, L, Woetmann, A, Koralov, SB, Bjarnsholt, T, Frieling, J, Schmelcher, M & Ødum, N 2023, 'Endolysin Inhibits Skin Colonization by Patient-Derived Staphylococcus Aureus and Malignant T-Cell Activation in Cutaneous T-Cell Lymphoma', Journal of Investigative Dermatology, vol. 143, no. 9, pp. 1757-1769. https://doi.org/10.1016/j.jid.2023.01.039

APA

Pallesen, E. M. H., Gluud, M., Vadivel, C. K., Buus, T. B., de Rooij, B., Zeng, Z., Ahmad, S., Willerslev-Olsen, A., Röhrig, C., Kamstrup, M. R., Bay, L., Lindahl, L., Krejsgaard, T., Geisler, C., Bonefeld, C. M., Iversen, L., Woetmann, A., Koralov, S. B., Bjarnsholt, T., ... Ødum, N. (2023). Endolysin Inhibits Skin Colonization by Patient-Derived Staphylococcus Aureus and Malignant T-Cell Activation in Cutaneous T-Cell Lymphoma. Journal of Investigative Dermatology, 143(9), 1757-1769. https://doi.org/10.1016/j.jid.2023.01.039

Vancouver

Pallesen EMH, Gluud M, Vadivel CK, Buus TB, de Rooij B, Zeng Z et al. Endolysin Inhibits Skin Colonization by Patient-Derived Staphylococcus Aureus and Malignant T-Cell Activation in Cutaneous T-Cell Lymphoma. Journal of Investigative Dermatology. 2023;143(9):1757-1769. https://doi.org/10.1016/j.jid.2023.01.039

Author

Pallesen, Emil M.H. ; Gluud, Maria ; Vadivel, Chella Krishna ; Buus, Terkild B. ; de Rooij, Bob ; Zeng, Ziao ; Ahmad, Sana ; Willerslev-Olsen, Andreas ; Röhrig, Christian ; Kamstrup, Maria R. ; Bay, Lene ; Lindahl, Lise ; Krejsgaard, Thorbjørn ; Geisler, Carsten ; Bonefeld, Charlotte M. ; Iversen, Lars ; Woetmann, Anders ; Koralov, Sergei B. ; Bjarnsholt, Thomas ; Frieling, Johan ; Schmelcher, Mathias ; Ødum, Niels. / Endolysin Inhibits Skin Colonization by Patient-Derived Staphylococcus Aureus and Malignant T-Cell Activation in Cutaneous T-Cell Lymphoma. In: Journal of Investigative Dermatology. 2023 ; Vol. 143, No. 9. pp. 1757-1769.

Bibtex

@article{6ff67a27dfa24a16abd25a3fff996542,
title = "Endolysin Inhibits Skin Colonization by Patient-Derived Staphylococcus Aureus and Malignant T-Cell Activation in Cutaneous T-Cell Lymphoma",
abstract = "Staphylococcus aureus is suspected to fuel disease activity in cutaneous T-cell lymphomas. In this study, we investigate the effect of a recombinant, antibacterial protein, endolysin (XZ.700), on S. aureus skin colonization and malignant T-cell activation. We show that endolysin strongly inhibits the proliferation of S. aureus isolated from cutaneous T-cell lymphoma skin and significantly decreases S. aureus bacterial cell counts in a dose-dependent manner. Likewise, ex vivo colonization of both healthy and lesional skin by S. aureus is profoundly inhibited by endolysin. Moreover, endolysin inhibits the patient-derived S. aureus induction of IFNγ and the IFNγ-inducible chemokine CXCL10 in healthy skin. Whereas patient-derived S. aureus stimulates activation and proliferation of malignant T cells in vitro through an indirect mechanism involving nonmalignant T cells, endolysin strongly inhibits the effects of S. aureus on activation (reduced CD25 and signal transducer and activator of transcription 5 phosphorylation) and proliferation (reduced Ki-67) of malignant T cells and cell lines in the presence of nonmalignant T cells. Taken together, we provide evidence that endolysin XZ.700 inhibits skin colonization, chemokine expression, and proliferation of pathogenic S. aureus and blocks their potential tumor-promoting effects on malignant T cells.",
author = "Pallesen, {Emil M.H.} and Maria Gluud and Vadivel, {Chella Krishna} and Buus, {Terkild B.} and {de Rooij}, Bob and Ziao Zeng and Sana Ahmad and Andreas Willerslev-Olsen and Christian R{\"o}hrig and Kamstrup, {Maria R.} and Lene Bay and Lise Lindahl and Thorbj{\o}rn Krejsgaard and Carsten Geisler and Bonefeld, {Charlotte M.} and Lars Iversen and Anders Woetmann and Koralov, {Sergei B.} and Thomas Bjarnsholt and Johan Frieling and Mathias Schmelcher and Niels {\O}dum",
note = "Publisher Copyright: {\textcopyright} 2023",
year = "2023",
doi = "10.1016/j.jid.2023.01.039",
language = "English",
volume = "143",
pages = "1757--1769",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "nature publishing group",
number = "9",

}

RIS

TY - JOUR

T1 - Endolysin Inhibits Skin Colonization by Patient-Derived Staphylococcus Aureus and Malignant T-Cell Activation in Cutaneous T-Cell Lymphoma

AU - Pallesen, Emil M.H.

AU - Gluud, Maria

AU - Vadivel, Chella Krishna

AU - Buus, Terkild B.

AU - de Rooij, Bob

AU - Zeng, Ziao

AU - Ahmad, Sana

AU - Willerslev-Olsen, Andreas

AU - Röhrig, Christian

AU - Kamstrup, Maria R.

AU - Bay, Lene

AU - Lindahl, Lise

AU - Krejsgaard, Thorbjørn

AU - Geisler, Carsten

AU - Bonefeld, Charlotte M.

AU - Iversen, Lars

AU - Woetmann, Anders

AU - Koralov, Sergei B.

AU - Bjarnsholt, Thomas

AU - Frieling, Johan

AU - Schmelcher, Mathias

AU - Ødum, Niels

N1 - Publisher Copyright: © 2023

PY - 2023

Y1 - 2023

N2 - Staphylococcus aureus is suspected to fuel disease activity in cutaneous T-cell lymphomas. In this study, we investigate the effect of a recombinant, antibacterial protein, endolysin (XZ.700), on S. aureus skin colonization and malignant T-cell activation. We show that endolysin strongly inhibits the proliferation of S. aureus isolated from cutaneous T-cell lymphoma skin and significantly decreases S. aureus bacterial cell counts in a dose-dependent manner. Likewise, ex vivo colonization of both healthy and lesional skin by S. aureus is profoundly inhibited by endolysin. Moreover, endolysin inhibits the patient-derived S. aureus induction of IFNγ and the IFNγ-inducible chemokine CXCL10 in healthy skin. Whereas patient-derived S. aureus stimulates activation and proliferation of malignant T cells in vitro through an indirect mechanism involving nonmalignant T cells, endolysin strongly inhibits the effects of S. aureus on activation (reduced CD25 and signal transducer and activator of transcription 5 phosphorylation) and proliferation (reduced Ki-67) of malignant T cells and cell lines in the presence of nonmalignant T cells. Taken together, we provide evidence that endolysin XZ.700 inhibits skin colonization, chemokine expression, and proliferation of pathogenic S. aureus and blocks their potential tumor-promoting effects on malignant T cells.

AB - Staphylococcus aureus is suspected to fuel disease activity in cutaneous T-cell lymphomas. In this study, we investigate the effect of a recombinant, antibacterial protein, endolysin (XZ.700), on S. aureus skin colonization and malignant T-cell activation. We show that endolysin strongly inhibits the proliferation of S. aureus isolated from cutaneous T-cell lymphoma skin and significantly decreases S. aureus bacterial cell counts in a dose-dependent manner. Likewise, ex vivo colonization of both healthy and lesional skin by S. aureus is profoundly inhibited by endolysin. Moreover, endolysin inhibits the patient-derived S. aureus induction of IFNγ and the IFNγ-inducible chemokine CXCL10 in healthy skin. Whereas patient-derived S. aureus stimulates activation and proliferation of malignant T cells in vitro through an indirect mechanism involving nonmalignant T cells, endolysin strongly inhibits the effects of S. aureus on activation (reduced CD25 and signal transducer and activator of transcription 5 phosphorylation) and proliferation (reduced Ki-67) of malignant T cells and cell lines in the presence of nonmalignant T cells. Taken together, we provide evidence that endolysin XZ.700 inhibits skin colonization, chemokine expression, and proliferation of pathogenic S. aureus and blocks their potential tumor-promoting effects on malignant T cells.

U2 - 10.1016/j.jid.2023.01.039

DO - 10.1016/j.jid.2023.01.039

M3 - Journal article

C2 - 36889662

AN - SCOPUS:85156222419

VL - 143

SP - 1757

EP - 1769

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 9

ER -

ID: 346595948