Flow cytometric analysis of RNA synthesis by detection of bromouridine incorporation

Research output: Contribution to journalJournal articleResearchpeer-review

RNA synthesis has traditionally been investigated by a laborious and time-consuming radiographic method involving incorporation of tritiated uridine. Now a faster non-radioactive alternative has emerged, based on immunocytochemical detection. This method utilizes the brominated RNA precursor bromouridine, which is taken into a cell, phosphorylated, and incorporated into nascent RNA. The BrU-substituted RNA is detected by permeabilizing the cells and staining with certain anti-BrdU antibodies. This dynamic approach yields information complementing that provided by cellular RNA content analysis at a given time and may be of value in studies of cellular activation and gene expression.
Original languageEnglish
JournalCurrent protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.]
VolumeChapter 7
Pages (from-to)Unit 7.12
Publication statusPublished - 2001

    Research areas

  • Animals, Antigens, Cell Membrane, Cell Nucleus, Cell Separation, Flow Cytometry, Gene Expression, Humans, RNA, Time Factors, Uridine

ID: 38517997