Flow cytometric measurement of RNA synthesis based on bromouridine labelling and combined with measurement of DNA content or cell surface antigen
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Flow cytometric measurement of RNA synthesis based on bromouridine labelling and combined with measurement of DNA content or cell surface antigen. / Jensen, P O; Larsen, J; Larsen, J K.
In: Acta Oncologica, Vol. 32, No. 5, 1993, p. 521-4.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Flow cytometric measurement of RNA synthesis based on bromouridine labelling and combined with measurement of DNA content or cell surface antigen
AU - Jensen, P O
AU - Larsen, J
AU - Larsen, J K
PY - 1993
Y1 - 1993
N2 - RNA synthesis can be analysed in nuclei or cells labelled with 5-bromouridine (BrUrd) and stained using cross-reacting anti-bromodeoxyuridine (BrdUrd) antibody. Flow cytometric dual parameter analysis of BrUrd incorporation and DNA content in nuclear suspensions of human blood lymphocytes showed that RNA synthesis increased within the first 24 hours of phytohemagglutinin (PHA) stimulation, reaching a maximum at 48 hours, when cells had entered the cell cycle. Using a new method for flow cytometric dual parameter analysis of BrUrd incorporation and a cell surface antigen, spontaneous RNA synthesis in HL-60 and K-562 cells was measured simultaneous with CD13 expression.
AB - RNA synthesis can be analysed in nuclei or cells labelled with 5-bromouridine (BrUrd) and stained using cross-reacting anti-bromodeoxyuridine (BrdUrd) antibody. Flow cytometric dual parameter analysis of BrUrd incorporation and DNA content in nuclear suspensions of human blood lymphocytes showed that RNA synthesis increased within the first 24 hours of phytohemagglutinin (PHA) stimulation, reaching a maximum at 48 hours, when cells had entered the cell cycle. Using a new method for flow cytometric dual parameter analysis of BrUrd incorporation and a cell surface antigen, spontaneous RNA synthesis in HL-60 and K-562 cells was measured simultaneous with CD13 expression.
KW - Antigens, CD
KW - Antigens, CD13
KW - Antigens, Differentiation, Myelomonocytic
KW - Antigens, Neoplasm
KW - Antigens, Surface
KW - Cell Cycle
KW - DNA, Neoplasm
KW - Flow Cytometry
KW - Humans
KW - Leukemia
KW - RNA, Neoplasm
KW - Tumor Cells, Cultured
KW - Uridine
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
M3 - Journal article
C2 - 8105832
VL - 32
SP - 521
EP - 524
JO - Acta Oncologica
JF - Acta Oncologica
SN - 1100-1704
IS - 5
ER -
ID: 181874043