Flow cytometric measurement of RNA synthesis based on bromouridine labelling and combined with measurement of DNA content or cell surface antigen

Research output: Contribution to journal › Journal article › Research › peer-review

RNA synthesis can be analysed in nuclei or cells labelled with 5-bromouridine (BrUrd) and stained using cross-reacting anti-bromodeoxyuridine (BrdUrd) antibody. Flow cytometric dual parameter analysis of BrUrd incorporation and DNA content in nuclear suspensions of human blood lymphocytes showed that RNA synthesis increased within the first 24 hours of phytohemagglutinin (PHA) stimulation, reaching a maximum at 48 hours, when cells had entered the cell cycle. Using a new method for flow cytometric dual parameter analysis of BrUrd incorporation and a cell surface antigen, spontaneous RNA synthesis in HL-60 and K-562 cells was measured simultaneous with CD13 expression.

Original languageEnglish
JournalActa Oncologica
Volume32
Issue number5
Pages (from-to)521-4
Number of pages4
ISSN0284-186X
Publication statusPublished - 1993

    Research areas

  • Antigens, CD, Antigens, CD13, Antigens, Differentiation, Myelomonocytic, Antigens, Neoplasm, Antigens, Surface, Cell Cycle, DNA, Neoplasm, Flow Cytometry, Humans, Leukemia, RNA, Neoplasm, Tumor Cells, Cultured, Uridine, Journal Article, Research Support, Non-U.S. Gov't

ID: 181874043