Imaging N-acyl homoserine lactone quorum sensing in vivo
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
In order to study N-acyl homoserine lactone (AHL)-based quorum sensing in vivo, we present a protocol using an Escherichia coli strain equipped with a luxR-based monitor system, which in the presence of exogenous AHL molecules expresses a green fluorescent protein (GFP). Lungs from mice challenged intratracheally with alginate beads containing both a Pseudomonas aeruginosa strain together with the E. coli monitor strain can be investigated at different time points postinfection. Epifluorescent or confocal scanning laser microscopy (CSLM) is used to detect the GFP-expressing E. coli monitor strain in the lung tissues, indicating production and excretion of AHLs in vivo by the infecting P. aeruginosa.
Original language | English |
---|---|
Title of host publication | Quorum Sensing : Methods and Protocols |
Editors | Livia Leoni, Giordano Rampion |
Number of pages | 10 |
Volume | 1673 |
Publisher | Humana Press |
Publication date | 2018 |
Pages | 203-212 |
ISBN (Print) | 978-1-4939-7308-8 |
ISBN (Electronic) | 978-1-4939-7309-5 |
DOIs | |
Publication status | Published - 2018 |
Series | Methods in Molecular Biology |
---|---|
Number | 1673 |
ISSN | 1064-3745 |
- CSLM, Epifluorescence microscopy, In vivo, Pseudomonas aeruginosa, Pulmonary infection model, Quorum sensing
Research areas
ID: 186872679